Technology

A New-Generation Label-Free Flow Cytometer

We’re developing a new-generation label-free flow cytometer, similar to conventional flow cytometry but based on electrical impedance cytometry. Instead of using biomarkers and lasers, our approach involves applying low AC voltage signals simultaneously at different frequencies.

Each compartment in a cell has a specific electric signature, and we recognize those. Our proprietary microfluidic device technology allows us to operate at very high frequencies, so that we can measure any change in the biophysical properties for the whole cell.

Users conduct effortless label-free measurements, generating rich data with diverse applications, from size measurements to cell cycle monitoring. The collected data are easily accessible, allowing users to revisit those at any time for exploring different cell properties.

new gen flow cytometry

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Hardware

Simple to operate and compact hardware, which is suitable for sterile operation.

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No need for labor intensive sample preparation. Only requirement: Resuspend cells in (physiological) buffer.
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Technology

Microfluidic flow cell, which is similar to conventional flow cytometers.

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Data

Rich data generation for a number of applications.​

conventional flow cytometry

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Hardware

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Preparation

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Technology

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data

Data

Workflow

Fast, sensitive & label-free

Our innovative flow cytometry system streamlines the conventional workflow by eliminating sample preparation and manual adjustments. Users can directly load samples without pipetting or calibration, simply starting the process with the push of a button. The system operates autonomously, conducting automated post-processing tasks such as cell event identification and clustering. Importantly, it does not require trained personnel for decision-making. The fully label-free operation, with applied voltages below electroporation limits, ensures cell integrity during measurements. This allows repeated measurements of the same sample throughout the experiment, thus eliminates the need for extra control samples. The system maintains user engagement by keeping them informed about the sample without requiring manual intervention.

 typical cytometry workflow
 InnoFluidics’ new generation cytometry workflow